論文 - 詳細
RRC ID | 32797 |
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著者 | Sasaki K, Ito T, Nishino N, Khochbin S, Yoshida M. |
タイトル | Real-time imaging of histone H4 hyperacetylation in living cells. |
ジャーナル | Proc Natl Acad Sci U S A |
Abstract |
To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors. |
巻・号 | 106(38) |
ページ | 16257-62 |
公開日 | 2009-9-22 |
DOI | 10.1073/pnas.0902150106 |
PII | 0902150106 |
PMID | 19805290 |
PMC | PMC2752582 |
MeSH | Acetylation / drug effects Animals COS Cells Chlorocebus aethiops Enzyme Inhibitors / pharmacology Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HeLa Cells Histone Deacetylase Inhibitors Histone Deacetylases / metabolism Histones / genetics Histones / metabolism* Humans Hydroxamic Acids / pharmacology Immunoblotting Lysine / genetics Lysine / metabolism* Microscopy, Confocal Microscopy, Fluorescence Mitosis Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Time Factors Transfection |
IF | 9.412 |
引用数 | 85 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
遺伝子材料 | Histac pcDNA3.1 (RDB12840) |
ヒト・動物細胞 | COS-7(RCB0539) |