論文 - 詳細
| RRC ID | 32798 |
|---|---|
| 著者 | Yamamoto S, Niida S, Azuma E, Yanagibashi T, Muramatsu M, Huang TT, Sagara H, Higaki S, Ikutani M, Nagai Y, Takatsu K, Miyazaki K, Hamashima T, Mori H, Matsuda N, Ishii Y, Sasahara M. |
| タイトル | Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes. |
| ジャーナル | Sci Rep |
| Abstract |
Emerging lines of evidence have shown that extracellular vesicles (EVs) mediate cell-to-cell communication by exporting encapsulated materials, such as microRNAs (miRNAs), to target cells. Endothelial cell-derived EVs (E-EVs) are upregulated in circulating blood in different pathological conditions; however, the characteristics and the role of these E-EVs are not yet well understood. In vitro studies were conducted to determine the role of inflammation-induced E-EVs in the cell-to-cell communication between vascular endothelial cells and pericytes/vSMCs. Stimulation with inflammatory cytokines and endotoxin immediately induced release of shedding type E-EVs from the vascular endothelial cells, and flow cytometry showed that the induction was dose dependent. MiRNA array analyses revealed that group of miRNAs were specifically increased in the inflammation-induced E-EVs. E-EVs added to the culture media of cerebrovascular pericytes were incorporated into the cells. The E-EV-supplemented cells showed highly induced mRNA and protein expression of VEGF-B, which was assumed to be a downstream target of the miRNA that was increased within the E-EVs after inflammatory stimulation. The results suggest that E-EVs mediate inflammation-induced endothelial cell-pericyte/vSMC communication, and the miRNAs encapsulated within the E-EVs may play a role in regulating target cell function. E-EVs may be new therapeutic targets for the treatment of inflammatory diseases. |
| 巻・号 | 5 |
| ページ | 8505 |
| 公開日 | 2015-2-17 |
| DOI | 10.1038/srep08505 |
| PII | srep08505 |
| PMID | 25687367 |
| PMC | PMC4330530 |
| MeSH | Animals Cell Communication Endothelial Cells / metabolism* Extracellular Vesicles / metabolism* Gene Expression Profiling Inflammation / genetics Inflammation / metabolism* Mice MicroRNAs / genetics Pericytes / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 3.998 |
| 引用数 | 64 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | GNP Clone IRAL020F10 (HGY088130) |