RRC ID 3283
著者 Maeda S, Ito M, Ando T, Ishimoto Y, Fujisawa Y, Takahashi H, Matsuda A, Sawamura A, Kato S.
タイトル Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.
ジャーナル FEMS Microbiol Lett
Abstract We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 24-48 h at the frequency of 10(-10)-10(-9) per recipient cell. PCR analysis of the transformed cells demonstrated the occurrence of lateral plasmid transfer. These cells survived until at least day 7 under antibiotic-free conditions. Liquid cultures of the same strains in Luria-Bertani broth produced no or few transformants, suggesting the importance of colony-biofilm formation for plasmid transfer. This is a novel line of evidence indicating that nonconjugative, nonviral horizontal gene transfer can occur between E. coli cells.
巻・号 255(1)
ページ 115-20
公開日 2006-2-1
DOI 10.1111/j.1574-6968.2005.00072.x
PII FML072
PMID 16436070
MeSH Biofilms / growth & development* Culture Media Escherichia coli / genetics* Escherichia coli / growth & development Gene Transfer, Horizontal* Plasmids / genetics* Transformation, Bacterial / genetics*
IF 1.987
引用数 43
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8788(CAG18439) ME8266 ME8584(KF1225) pHSG299 pGBM1