RRC ID |
32838
|
著者 |
Zhu H, Yoshimoto T, Imajo-Ohmi S, Dazortsava M, Mathivanan A, Yamashima T.
|
タイトル |
Why are hippocampal CA1 neurons vulnerable but motor cortex neurons resistant to transient ischemia?
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ジャーナル |
J Neurochem
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Abstract |
It is well-known that heat-shock protein 70.1 (Hsp70.1), a major protein of the human Hsp70 family, plays cytoprotective roles by both its chaperone function and stabilization of lysosomal membranes. Recently, we found that calpain-mediated cleavage of carbonylated Hsp70.1 in the hippocampal cornu Ammonis1 (CA1) contributes to neuronal death after transient global ischemia. This study aims to elucidate the differential neuronal vulnerability between the motor cortex and CA1 sector against ischemia/reperfusion. Fluoro-Jade B staining and terminal deoxynucleotidyl transferase-mediated dUTP-nick-end-labeling analysis of the monkey brain undergoing 20min whole brain ischemia followed by reperfusion, showed that the motor cortex is significantly resistant to the ischemic insult compared with CA1. Up-regulation of Hsp70.1 but absence of its cleavage by calpain facilitated its binding with NF-κB p65/IκBα complex to minimize NF-κB p65 activation, which contributed to a neuroprotection in the motor cortex. In contrast, because activated μ-calpain cleaved carbonylated Hsp70.1 in CA1, the resultant Hsp70.1 dysfunction not only destabilized lysosomal membrane but also induced a sustained activation of NF-κB p65, both of which resulted in delayed neuronal death. We propose that the cascades underlying lysosomal stabilization and regulating NF-κB activation by Hsp70.1 may influence neuronal survival/death after the ischemia/reperfusion.
|
巻・号 |
120(4)
|
ページ |
574-85
|
公開日 |
2012-2-1
|
DOI |
10.1111/j.1471-4159.2011.07550.x
|
PMID |
22017466
|
MeSH |
Animals
CA1 Region, Hippocampal / blood supply
CA1 Region, Hippocampal / metabolism*
CA1 Region, Hippocampal / pathology*
Cell Death / physiology
HSP70 Heat-Shock Proteins / physiology*
Ischemic Attack, Transient / metabolism*
Ischemic Attack, Transient / pathology*
Macaca
Motor Cortex / blood supply
Motor Cortex / metabolism*
Motor Cortex / pathology*
Neurons / metabolism
Neurons / pathology*
Protein Binding / physiology
|
IF |
4.066
|
引用数 |
29
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NEUROSCIENCES
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リソース情報 |
ニホンザル |
|