RRC ID |
3288
|
著者 |
Nagashima K, Kubota Y, Shibata T, Sakaguchi C, Shinagawa H, Hishida T.
|
タイトル |
Degradation of Escherichia coli RecN aggregates by ClpXP protease and its implications for DNA damage tolerance.
|
ジャーナル |
J Biol Chem
|
Abstract |
Protein degradation in bacteria plays a dynamic and critical role in the cellular response to environmental stimuli such as heat shock and DNA damage and in removing damaged proteins or protein aggregates. Escherichia coli recN is a member of the structural maintenance of chromosomes family and is required for DNA double strand break (DSB) repair. This study shows that RecN protein has a short half-life and its degradation is dependent on the cytoplasmic protease ClpXP and a degradation signal at the C terminus of RecN. In cells with DNA DSBs, green fluorescent protein-RecN localized in discrete foci on nucleoids and formed visible aggregates in the cytoplasm, both of which disappeared rapidly in wild-type cells when DSBs were repaired. In contrast, in DeltaclpX cells, RecN aggregates persisted in the cytoplasm after release from DNA damage. Furthermore, analysis of cells experiencing chronic DNA damage revealed that proteolytic removal of RecN aggregates by ClpXP was important for cell viability. These data demonstrate that ClpXP is a critical factor in the cellular clearance of cytoplasmic RecN aggregates from the cell and therefore plays an important role in DNA damage tolerance.
|
巻・号 |
281(41)
|
ページ |
30941-6
|
公開日 |
2006-10-13
|
DOI |
10.1074/jbc.M606566200
|
PII |
S0021-9258(19)89308-0
|
PMID |
16914543
|
MeSH |
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Cytoplasm / enzymology
Cytoplasm / metabolism
DNA Damage*
DNA Repair
DNA Restriction Enzymes / chemistry*
DNA Restriction Enzymes / metabolism
Endopeptidase Clp / metabolism*
Escherichia coli / enzymology*
Escherichia coli / metabolism
Escherichia coli Proteins / metabolism*
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Mitomycin / pharmacology
Nucleic Acid Synthesis Inhibitors / pharmacology
Plasmids / metabolism
Protein Structure, Tertiary
Time Factors
|
IF |
4.238
|
引用数 |
24
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
CV-12(BW25141) |