RRC ID |
330
|
著者 |
Nishihara S, Ueda R, Goto S, Toyoda H, Ishida H, Nakamura M.
|
タイトル |
Approach for functional analysis of glycan using RNA interference.
|
ジャーナル |
Glycoconj J
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Abstract |
The elucidation of the biological role of glycan is one of the most important issues to be resolved following the genome project. RNA interference is becoming an efficient reverse genetic tool for studying gene function in model organisms, including C.elegans and Drosophila melanogaster. Our molecular evolutionary study has shown that a prototype of glycosyltransferases, which synthesize a variety of glycan structures in the Golgi apparatus, was conserved between mammals and Drosophila. For analyses of the basic physiological functions of glycans, we established the Drosophila inducible RNAi knockdown system and applied it to one glycosyltransferase and one transporter, proteoglycan UDP-galactose: beta-xylose beta1,4galactosyltransferase I and the PAPS-transporter, respectively. If on the silencing of each gene induced ubiquitously under the control of a cytoplasmic actin promoter, the RNAi knockdown fly died, then the protein was indispensable for life. The expression of the target gene was disrupted specifically and the degree of interference was well correlated with the phenotype. The inducible RNAi knockdown fly obtained using the GAL4-UAS system will pave the way for the functional analysis of glycans.
|
巻・号 |
21(1-2)
|
ページ |
63-8
|
公開日 |
2004-8-1
|
DOI |
10.1023/B:GLYC.0000043750.80389.14
|
PII |
5277453
|
PMID |
15467401
|
MeSH |
Actins / genetics
Animals
Biological Transport
Caenorhabditis elegans
Carbohydrates / chemistry
Cytoplasm / metabolism
Databases as Topic
Drosophila melanogaster
Golgi Apparatus / metabolism
Models, Genetic
Phylogeny
Polysaccharides / chemistry*
Promoter Regions, Genetic
RNA Interference*
RNA, Messenger / metabolism
Substrate Specificity
|
IF |
2.197
|
引用数 |
8
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ショウジョウバエ |
|