論文 - 詳細
RRC ID | 3300 |
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著者 | Yamamoto K, Ishihama A. |
タイトル | Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)(4-8)GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes. |
巻・号 | 70(7) |
ページ | 1688-95 |
公開日 | 2006-7-1 |
DOI | 10.1271/bbb.60024 |
PII | JST.JSTAGE/bbb/60024 |
PMID | 16861804 |
MeSH | Base Sequence Copper / pharmacology* DNA Footprinting Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Molecular Sequence Data Mutation Promoter Regions, Genetic* Regulon* |
IF | 1.516 |
引用数 | 96 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
原核生物(大腸菌) | JW1832 |