Reference - Detail
| RRC ID | 33036 |
|---|---|
| Author | Vinayagam A, Zirin J, Roesel C, Hu Y, Yilmazel B, Samsonova AA, Neumüller RA, Mohr SE, Perrimon N. |
| Title | Integrating protein-protein interaction networks with phenotypes reveals signs of interactions. |
| Journal | Nat Methods |
| Abstract |
A major objective of systems biology is to organize molecular interactions as networks and to characterize information flow within networks. We describe a computational framework to integrate protein-protein interaction (PPI) networks and genetic screens to predict the 'signs' of interactions (i.e., activation-inhibition relationships). We constructed a Drosophila melanogaster signed PPI network consisting of 6,125 signed PPIs connecting 3,352 proteins that can be used to identify positive and negative regulators of signaling pathways and protein complexes. We identified an unexpected role for the metabolic enzymes enolase and aldo-keto reductase as positive and negative regulators of proteolysis, respectively. Characterization of the activation-inhibition relationships between physically interacting proteins within signaling pathways will affect our understanding of many biological functions, including signal transduction and mechanisms of disease. |
| Volume | 11(1) |
| Pages | 94-9 |
| Published | 2014-1-1 |
| DOI | 10.1038/nmeth.2733 |
| PII | nmeth.2733 |
| PMID | 24240319 |
| PMC | PMC3877743 |
| MeSH | Alcohol Oxidoreductases / metabolism Aldehyde Reductase Aldo-Keto Reductases Animals Computational Biology / methods Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation Phenotype Proteasome Endopeptidase Complex / chemistry Protein Interaction Mapping* Protein Interaction Maps Proteins / metabolism RNA Interference RNA, Double-Stranded / metabolism Signal Transduction Systems Biology / methods |
| IF | 30.822 |
| Times Cited | 76 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 17655R-1 17654R-2 32101R-2 32101R-3 |