RRC ID 33067
Author Huang YC, Lu YN, Wu JT, Chien CT, Pi H.
Title The COP9 signalosome converts temporal hormone signaling to spatial restriction on neural competence.
Journal PLoS Genet.
Abstract During development, neural competence is conferred and maintained by integrating spatial and temporal regulations. The Drosophila sensory bristles that detect mechanical and chemical stimulations are arranged in stereotypical positions. The anterior wing margin (AWM) is arrayed with neuron-innervated sensory bristles, while posterior wing margin (PWM) bristles are non-innervated. We found that the COP9 signalosome (CSN) suppresses the neural competence of non-innervated bristles at the PWM. In CSN mutants, PWM bristles are transformed into neuron-innervated, which is attributed to sustained expression of the neural-determining factor Senseless (Sens). The CSN suppresses Sens through repression of the ecdysone signaling target gene broad (br) that encodes the BR-Z1 transcription factor to activate sens expression. Strikingly, CSN suppression of BR-Z1 is initiated at the prepupa-to-pupa transition, leading to Sens downregulation, and termination of the neural competence of PWM bristles. The role of ecdysone signaling to repress br after the prepupa-to-pupa transition is distinct from its conventional role in activation, and requires CSN deneddylating activity and multiple cullins, the major substrates of deneddylation. Several CSN subunits physically associate with ecdysone receptors to represses br at the transcriptional level. We propose a model in which nuclear hormone receptors cooperate with the deneddylation machinery to temporally shutdown downstream target gene expression, conferring a spatial restriction on neural competence at the PWM.
Volume 10(11)
Pages e1004760
Published 2014-11
DOI 10.1371/journal.pgen.1004760
PMID 25393278
PMC PMC4230841
MeSH Animals COP9 Signalosome Complex Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Ecdysone / genetics Ecdysone / metabolism Gene Expression Regulation, Developmental Multiprotein Complexes / genetics* Multiprotein Complexes / metabolism Mutation Neurons / metabolism Nuclear Proteins / genetics* Nuclear Proteins / metabolism Peptide Hydrolases / genetics* Peptide Hydrolases / metabolism Transcription Factors / genetics* Transcription Factors / metabolism Wings, Animal / growth & development*
IF 5.54
Times Cited 2
Drosophila 32120R-2