RRC ID 33072
Author Chen XJ, Squarr AJ, Stephan R, Chen B, Higgins TE, Barry DJ, Martin MC, Rosen MK, Bogdan S, Way M.
Title Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.
Journal Dev Cell
Abstract Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motility by virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the Ena/VASP EVH1 domain with an extended proline rich motif in Abi. This interaction increases cell migration and enables VASP to cooperatively enhance WRC stimulation of Arp2/3 complex-mediated actin assembly in vitro in the presence of Rac. Loss of this interaction in Drosophila macrophages results in defects in lamellipodia formation, cell spreading, and redistribution of Ena to the tips of filopodia-like extensions. Rescue experiments of abi mutants also reveals a physiological requirement for the Abi:Ena interaction in photoreceptor axon targeting and oogenesis. Our data demonstrate that the activities of Ena/VASP and the WRC are intimately linked to ensure optimal control of actin polymerization during cell migration and development.
Volume 30(5)
Pages 569-84
Published 2014-9-8
DOI 10.1016/j.devcel.2014.08.001
PII S1534-5807(14)00493-6
PMID 25203209
PMC PMC4165403
MeSH Actin Cytoskeleton / metabolism* Amino Acid Sequence Animals Axons / physiology Cell Line, Tumor Cell Movement DNA-Binding Proteins / metabolism* Drosophila melanogaster Gene Expression Regulation, Developmental* Humans Macrophages / metabolism Molecular Sequence Data Photoreceptor Cells, Invertebrate / metabolism Proline / chemistry Protein Structure, Tertiary Pseudopodia / metabolism Sequence Homology, Amino Acid Wiskott-Aldrich Syndrome Protein Family / metabolism*
IF 10.092
Times Cited 44
Drosophila 4636R-1 4636R-2