| RRC ID |
33072
|
| 著者 |
Chen XJ, Squarr AJ, Stephan R, Chen B, Higgins TE, Barry DJ, Martin MC, Rosen MK, Bogdan S, Way M.
|
| タイトル |
Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motility by virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the Ena/VASP EVH1 domain with an extended proline rich motif in Abi. This interaction increases cell migration and enables VASP to cooperatively enhance WRC stimulation of Arp2/3 complex-mediated actin assembly in vitro in the presence of Rac. Loss of this interaction in Drosophila macrophages results in defects in lamellipodia formation, cell spreading, and redistribution of Ena to the tips of filopodia-like extensions. Rescue experiments of abi mutants also reveals a physiological requirement for the Abi:Ena interaction in photoreceptor axon targeting and oogenesis. Our data demonstrate that the activities of Ena/VASP and the WRC are intimately linked to ensure optimal control of actin polymerization during cell migration and development.
|
| 巻・号 |
30(5)
|
| ページ |
569-84
|
| 公開日 |
2014-9-8
|
| DOI |
10.1016/j.devcel.2014.08.001
|
| PII |
S1534-5807(14)00493-6
|
| PMID |
25203209
|
| PMC |
PMC4165403
|
| MeSH |
Actin Cytoskeleton / metabolism*
Amino Acid Sequence
Animals
Axons / physiology
Cell Line, Tumor
Cell Movement
DNA-Binding Proteins / metabolism*
Drosophila melanogaster
Gene Expression Regulation, Developmental*
Humans
Macrophages / metabolism
Molecular Sequence Data
Photoreceptor Cells, Invertebrate / metabolism
Proline / chemistry
Protein Structure, Tertiary
Pseudopodia / metabolism
Sequence Homology, Amino Acid
Wiskott-Aldrich Syndrome Protein Family / metabolism*
|
| IF |
10.092
|
| 引用数 |
44
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
4636R-1
4636R-2 |
