論文 - 詳細
| RRC ID | 33096 |
|---|---|
| 著者 | Dudin O, Bendezú FO, Groux R, Laroche T, Seitz A, Martin SG. |
| タイトル | A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast. |
| ジャーナル | J Cell Biol |
| Abstract |
Cell-cell fusion is essential for fertilization. For fusion of walled cells, the cell wall must be degraded at a precise location but maintained in surrounding regions to protect against lysis. In fission yeast cells, the formin Fus1, which nucleates linear actin filaments, is essential for this process. In this paper, we show that this formin organizes a specific actin structure-the actin fusion focus. Structured illumination microscopy and live-cell imaging of Fus1, actin, and type V myosins revealed an aster of actin filaments whose barbed ends are focalized near the plasma membrane. Focalization requires Fus1 and type V myosins and happens asynchronously always in the M cell first. Type V myosins are essential for fusion and concentrate cell wall hydrolases, but not cell wall synthases, at the fusion focus. Thus, the fusion focus focalizes cell wall dissolution within a broader cell wall synthesis zone to shift from cell growth to cell fusion. |
| 巻・号 | 208(7) |
| ページ | 897-911 |
| 公開日 | 2015-3-30 |
| DOI | 10.1083/jcb.201411124 |
| PII | jcb.201411124 |
| PMID | 25825517 |
| PMC | PMC4384723 |
| MeSH | Actin Cytoskeleton / metabolism Actins / metabolism Cell Fusion Cell Membrane / metabolism Cell Wall / metabolism* Cytoskeletal Proteins / metabolism Hydrolases / metabolism* Myosin Type V / metabolism* Myosins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 8.811 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | S. bombe strains |