RRC ID 33101
Author Shenton MR, Ohyanagi H, Wang ZX, Toyoda A, Fujiyama A, Nagata T, Feng Q, Han B, Kurata N.
Title Rapid turnover of antimicrobial-type cysteine-rich protein genes in closely related Oryza genomes.
Journal Mol Genet Genomics
Abstract Defensive and reproductive protein genes undergo rapid evolution. Small, cysteine-rich secreted peptides (CRPs) act as antimicrobial agents and function in plant intercellular signaling and are over-represented among reproductively expressed proteins. Because of their roles in defense, reproduction and development and their presence in multigene families, CRP variation can have major consequences for plant phenotypic and functional diversification. We surveyed the CRP genes of six closely related Oryza genomes comprising Oryza sativa ssp. japonica and ssp. indica, Oryza glaberrima and three accessions of Oryza rufipogon to observe patterns of evolution in these gene families and the effects of variation on their gene expression. These Oryza genomes, like other plant genomes, have accumulated large reservoirs of CRP sequences, comprising 26 groups totaling between 676 and 843 genes, in contrast to antimicrobial CRPs in animal genomes. Despite the close evolutionary relationships between the genomes, we observed rapid changes in number and structure among CRP gene families. Many CRP sequences are in gene clusters generated by local duplications, have undergone rapid turnover and are more likely to be silent or specifically expressed. By contrast, conserved CRP genes are more likely to be highly and broadly expressed. Variable CRP genes created by repeated duplication, gene modification and inactivation can gain new functions and expression patterns in newly evolved gene copies. For the CRP proteins, the process of gain/loss by deletion or duplication at gene clusters seems to be an important mechanism in evolution of the gene families, which also contributes to their expression evolution.
Volume 290(5)
Pages 1753-70
Published 2015-10-1
DOI 10.1007/s00438-015-1028-4
PII 10.1007/s00438-015-1028-4
PMID 25842177
MeSH Anti-Infective Agents Cysteine / metabolism* Gene Expression Regulation, Plant Genes, Plant* Oryza / genetics* Plant Proteins / genetics* Plant Proteins / metabolism Polymorphism, Genetic Sequence Analysis, RNA Species Specificity
IF 2.879
Times Cited 5
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Rice W0106 W1921 W1943