RRC ID 3311
Author Gerding MA, Ogata Y, Pecora ND, Niki H, de Boer PA.
Title The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli.
Journal Mol. Microbiol.
Abstract Fission of bacterial cells involves the co-ordinated invagination of the envelope layers. Invagination of the cytoplasmic membrane (IM) and peptidoglycan (PG) layer is likely driven by the septal ring organelle. Invagination of the outer membrane (OM) in Gram-negative species is thought to occur passively via its tethering to the underlying PG layer with generally distributed PG-binding OM (lipo)proteins. The Tol-Pal system is energized by proton motive force and is well conserved in Gram-negative bacteria. It consists of five proteins that can connect the OM to both the PG and IM layers via protein-PG and protein-protein interactions. Although the system is needed to maintain full OM integrity, and for class A colicins and filamentous phages to enter cells, its precise role has remained unclear. We show that all five components accumulate at constriction sites in Escherichia coli and that mutants lacking an intact system suffer delayed OM invagination and contain large OM blebs at constriction sites and cell poles. We propose that Tol-Pal constitutes a dynamic subcomplex of the division apparatus in Gram-negative bacteria that consumes energy to establish transient trans-envelope connections at/near the septal ring to draw the OM onto the invaginating PG and IM layers during constriction.
Volume 63(4)
Pages 1008-25
Published 2007-2
DOI 10.1111/j.1365-2958.2006.05571.x
PII MMI5571
PMID 17233825
PMC PMC4428343
MeSH Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Base Sequence Cell Division Cytoplasm / metabolism Escherichia coli / cytology* Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Intracellular Membranes / metabolism Lipoproteins / genetics Lipoproteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Molecular Sequence Data Multiprotein Complexes Mutation Peptidoglycan / genetics Peptidoglycan / metabolism* Periplasmic Proteins / genetics Periplasmic Proteins / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 3.649
Times Cited 160
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY MICROBIOLOGY
Resource
Prokaryotes E. coli ASKA