| RRC ID |
33132
|
| 著者 |
Nojima Y, Ito K, Ono H, Nakazato T, Bono H, Yokoyama T, Sato R, Suetsugu Y, Nakamura Y, Yamamoto K, Satoh J, Tabunoki H, Fugo H.
|
| タイトル |
Superoxide dismutases, SOD1 and SOD2, play a distinct role in the fat body during pupation in silkworm Bombyx mori.
|
| ジャーナル |
PLoS One
|
| Abstract |
One way that aerobic biological systems counteract the generation of reactive oxygen species (ROS) is with superoxide dismutase proteins SOD1 and SOD2 that metabolize superoxide radicals to molecular oxygen and hydrogen peroxide or scavenge oxygen radicals produced by the extensive oxidation-reduction and electron-transport reactions that occur in mitochondria. We characterized SOD1 and SOD2 of Bombyx mori isolated from the fat body of larvae. Immunological analysis demonstrated the presence of BmSOD1 and BmSOD2 in the silk gland, midgut, fat body, Malpighian tubules, testis and ovary from larvae to adults. We found that BmSOD2 had a unique expression pattern in the fat body through the fifth instar larval developmental stage. The anti-oxidative functions of BmSOD1 and BmSOD2 were assessed by exposing larvae to insecticide rotenone or vasodilator isosorbide dinitrate, which is an ROS generator in BmN4 cells; however, exposure to these compounds had no effect on the expression levels of either BmSOD protein. Next, we investigated the physiological role of BmSOD1 and BmSOD2 under environmental oxidative stress, applied through whole-body UV irradiation and assayed using quantitative RT-PCR, immunoblotting and microarray analysis. The mRNA expression level of both BmSOD1 and BmSOD2 was markedly increased but protein expression level was increased only slightly. To examine the differences in mRNA and protein level due to UV irradiation intensity, we performed microarray analysis. Gene set enrichment analysis revealed that genes in the insulin signaling pathway and PPAR signaling pathway were significantly up-regulated after 6 and 12 hours of UV irradiation. Taken together, the activities of BmSOD1 and BmSOD2 may be related to the response to UV irradiation stress in B. mori. These results suggest that BmSOD1 and BmSOD2 modulate environmental oxidative stress in the cell and have a specific role in fat body of B. mori during pupation.
|
| 巻・号 |
10(2)
|
| ページ |
e0116007
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0116007
|
| PII |
PONE-D-14-25465
|
| PMID |
25714339
|
| PMC |
PMC4340916
|
| MeSH |
Amino Acid Sequence
Animals
Bombyx / drug effects
Bombyx / genetics
Bombyx / metabolism*
Cell Line
Cloning, Molecular
DNA, Complementary / genetics
Fat Body / metabolism*
Gene Expression Regulation
Insecticides / pharmacology
Isosorbide Dinitrate / pharmacology
Molecular Sequence Data
Organ Specificity
Phylogeny
Reactive Oxygen Species / metabolism
Rotenone / pharmacology
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism*
Superoxide Dismutase-1
|
| IF |
2.74
|
| 引用数 |
13
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
