論文 - 詳細
| RRC ID | 33138 |
|---|---|
| 著者 | Li Z, Mon H, Mitsunobu H, Zhu L, Xu J, Lee JM, Kusakabe T. |
| タイトル | Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
Polycomb group (PcG) complexes are known to be chromatin modifiers and transcriptional repressors. In this work, we reported that the histone-modifying PcG complexes are able to participate in the repair process of ultraviolet (UV)-induced DNA lesions in the silkworm, Bombyx mori. The silkworm cells with depletion of PcG genes showed hypersensitive to UV-C irradiation and increased inhibition of cell proliferation. Interestingly, an SQ site in the silkworm-human chimeric H2A protein synthesized here was phosphorylated rapidly upon UV-C exposure, which could be used as a marker for monitoring the response to DNA damage in silkworm cells. Under these UV-C irradiated conditions, we found that PRC1-mediated ubiquitylation of H2AX, but not of H2AZ, were decreased and this deubiquitylation was independent of its phosphorylation event. In contrast, UV-C irradiation induced the increase of trimethylation of lysine 27 on histone H3 (H3K27me3), a mark of transcriptionally silent chromatin catalyzed by another PcG subcomplex, PRC2. Collectively, we provided the first evidence on chromatin remodeling in response to UV-C lesion in silkworm and revealed another layer role for PcG complexes-mediated histone modifications in contributing to creating an open chromatin structure for the efficient repair of DNA damages. |
| 巻・号 | 55 |
| ページ | 9-18 |
| 公開日 | 2014-12-1 |
| DOI | 10.1016/j.ibmb.2014.10.001 |
| PII | S0965-1748(14)00161-1 |
| PMID | 25308962 |
| MeSH | Animals Bombyx / genetics* Bombyx / radiation effects* Cell Line Chromatin / chemistry Chromatin / radiation effects Chromatin Assembly and Disassembly / genetics DNA Damage / genetics* DNA Damage / radiation effects* Histones / metabolism* Humans Methylation Polycomb-Group Proteins / metabolism* Ubiquitination Ultraviolet Rays / adverse effects* |
| IF | 3.827 |
| 引用数 | 5 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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