| RRC ID |
33154
|
| 著者 |
Kobayashi N, Takahashi M, Kihara S, Niimi T, Yamashita O, Yaginuma T.
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| タイトル |
Cloning of cDNA encoding a Bombyx mori homolog of human oxidation resistance 1 (OXR1) protein from diapause eggs, and analyses of its expression and function.
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| ジャーナル |
J Insect Physiol
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| Abstract |
To better understand the molecular mechanisms of diapause initiation, we used the sensitive cDNA subtraction (selective amplification via biotin- and restriction-mediated enrichment) method and isolated a novel gene expressed abundantly in diapause eggs of the silkworm, Bombyx mori, which encodes a homolog of the human oxidation resistance 1 (OXR1) protein. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting analyses confirmed that BmOXR1 mRNA and its 140-kDa protein were differentially expressed in diapause eggs compared to non-diapause eggs. OXR1 double-stranded RNA (dsRNA) was injected into diapause-destined eggs before the cellular blastoderm stage, and 4days later, when untreated eggs reached the diapause stage, the OXR1 protein disappeared; however, these eggs remained in diapause, suggesting that BmOXR1 is not essential for diapause initiation and/or maintenance. To further investigate the in vivo function of BmOXR1 apart from its role in diapause, we overexpressed BmOXR1 in Drosophila melanogaster. The fruit fly male adult life-span was significantly extended in the 50%-survival time when adults were reared on diets both with and without H2O2 solution under 25°C incubation. These results suggest that BmOXR1 functions in D. melanogaster via a possible antioxidant effect. As BmOXR1 was expressed mainly in the nuclei of D. melanogaster cells, the mechanism underlying its antioxidation effect appears to be different from that in humans where it is expressed mainly in the mitochondria. Taken together, these results suggest that BmOXR1 might serve as an antioxidant regulator during the early diapause stage.
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| 巻・号 |
68
|
| ページ |
58-68
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| 公開日 |
2014-9-1
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| DOI |
10.1016/j.jinsphys.2014.06.020
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| PII |
S0022-1910(14)00135-8
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| PMID |
25010546
|
| MeSH |
Animals
Bombyx / embryology
Bombyx / genetics*
Bombyx / physiology
Cloning, Molecular*
DNA, Complementary / genetics
Diapause, Insect / physiology*
Drosophila melanogaster / physiology*
Hydrogen Peroxide / pharmacology
Insect Proteins / genetics
Insect Proteins / metabolism*
Longevity
Ovum
RNA, Double-Stranded
Real-Time Polymerase Chain Reaction
|
| IF |
2.246
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| 引用数 |
20
|
|
WOS 分野
|
ENTOMOLOGY
ZOOLOGY
PHYSIOLOGY
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| リソース情報 |
| カイコ |
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