論文 - 詳細
| RRC ID | 33155 |
|---|---|
| 著者 | Sakai H, Sakaguchi H, Aoki F, Suzuki MG. |
| タイトル | Functional analysis of sex-determination genes by gene silencing with LNA-DNA gapmers in the silkworm, Bombyx mori. |
| ジャーナル | Mech Dev |
| Abstract |
The sexual fate of B. mori is determined genetically; ZW, female and ZZ, male. Recently, we successfully identified a strong candidate gene at the top of the sex determination cascade in B. mori. This gene was termed Feminizer (Fem) and revealed to be a source of Fem-piRNA. Further, we found that B. mori doublesex (Bmdsx) splicing was markedly altered to produce the male-type isoform when a Fem-piRNA inhibitor was injected into ZW embryos. Moreover, knockdown of Masculinizer (Masc), a Fem-piRNA target gene, altered to produce the female-type isoform of Bmdsx in male embryos. However, it remains unclear as to whether Masc directly regulates the sex-specific expression of Bmdsx. In previous studies, we determined that the male-specific isoform of the Bombyx homolog of IGF-II mRNA-binding protein (Imp(M)) was involved in the male-specific splicing of Bmdsx. In an attempt to clarify the genetic relationship between Fem, Masc, Imp(M), and Bmdsx, knockdown experiments were performed. Knockdown of Fem shifted into male-type Bmdsx, Imp(M) and Masc in female embryos. Knockdown of Masc led to the production of the female-type Bmdsx and a dramatic reduction in Imp(M) expression in male embryos. Knockdown of Imp(M) shifted Bmdsx splice mode from the male-type into the female-type. Our results suggest that: (1) Fem reduces Masc expression, (2) Masc dramatically induces Imp(M) expression, and (3) Imp(M) shifting Bmdsx splice mode from the female-type into the male-type. Based on these findings, we propose a possible genetic cascade regulating sex determination in B. mori. |
| 巻・号 | 137 |
| ページ | 45-52 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.mod.2015.03.002 |
| PII | S0925-4773(15)00027-1 |
| PMID | 25828912 |
| MeSH | Animals Bombyx / genetics* DNA-Binding Proteins / genetics Female Gene Silencing / physiology* Insect Proteins / genetics* Male RNA Isoforms / genetics RNA Splicing / genetics* RNA, Messenger / genetics RNA, Small Interfering / genetics Sex Determination Processes / genetics* |
| IF | 2.126 |
| 引用数 | 6 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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