論文 - 詳細
| RRC ID | 33166 |
|---|---|
| 著者 | Satoh T, Ohba A, Liu Z, Inagaki T, Satoh AK. |
| タイトル | dPob/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors. |
| ジャーナル | Elife |
| Abstract |
In eukaryotes, most integral membrane proteins are synthesized, integrated into the membrane, and folded properly in the endoplasmic reticulum (ER). We screened the mutants affecting rhabdomeric expression of rhodopsin 1 (Rh1) in the Drosophila photoreceptors and found that dPob/EMC3, EMC1, and EMC8/9, Drosophila homologs of subunits of ER membrane protein complex (EMC), are essential for stabilization of immature Rh1 in an earlier step than that at which another Rh1-specific chaperone (NinaA) acts. dPob/EMC3 localizes to the ER and associates with EMC1 and calnexin. Moreover, EMC is required for the stable expression of other multi-pass transmembrane proteins such as minor rhodopsins Rh3 and Rh4, transient receptor potential, and Na(+)K(+)-ATPase, but not for a secreted protein or type I single-pass transmembrane proteins. Furthermore, we found that dPob/EMC3 deficiency induces rhabdomere degeneration in a light-independent manner. These results collectively indicate that EMC is a key factor in the biogenesis of multi-pass transmembrane proteins, including Rh1, and its loss causes retinal degeneration. |
| 巻・号 | 4 |
| 公開日 | 2015-2-26 |
| DOI | 10.7554/eLife.06306 |
| PMID | 25715730 |
| PMC | PMC4341237 |
| MeSH | Animals Drosophila / metabolism* Drosophila Proteins / metabolism* Membrane Proteins / metabolism* Photoreceptor Cells, Invertebrate / metabolism* Protein Transport Rhodopsin / biosynthesis* |
| IF | 7.08 |
| 引用数 | 50 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | P (RS3)[CB.0279.3] (DGRC#123106) y w ey-FLP CG6750[e02662] FRT40A/ CyO y[+] (DGRC#114504) |