論文 - 詳細
| RRC ID | 3318 |
|---|---|
| 著者 | Lee J, Page R, García-Contreras R, Palermino JM, Zhang XS, Doshi O, Wood TK, Peti W. |
| タイトル | Structure and function of the Escherichia coli protein YmgB: a protein critical for biofilm formation and acid-resistance. |
| ジャーナル | J Mol Biol |
| Abstract |
The Escherichia coli gene cluster ymgABC was identified in transcriptome studies to have a role in biofilm development and stability. In this study, we showed that YmgB represses biofilm formation in rich medium containing glucose, decreases cellular motility, and protects the cell from acid indicating that YmgB has a major role in acid-resistance in E. coli. Our data show that these phenotypes are potentially mediated through interactions with the important cell signal indole. In addition, gel mobility-shift assays suggest that YmgB may be a non-specific DNA-binding protein. Using nickel-enrichment DNA microarrays, we showed that YmgB binds, either directly or indirectly, via a probable ligand, genes important for biofilm formation. To advance our understanding of the function of YmgB, we used X-ray crystallography to solve the structure of the protein to 1.8 A resolution. YmgB is a biological dimer that is structurally homologous to the E. coli gene regulatory protein Hha, despite having only 5% sequence identity. This supports our DNA microarray data showing that YmgB is a gene regulatory protein. Therefore, this protein, which clearly has a critical role in acid-resistance in E. coli, has been renamed as AriR for regulator of acid resistance influenced by indole. |
| 巻・号 | 373(1) |
| ページ | 11-26 |
| 公開日 | 2007-10-12 |
| DOI | 10.1016/j.jmb.2007.07.037 |
| PII | S0022-2836(07)00964-3 |
| PMID | 17765265 |
| PMC | PMC2185545 |
| MeSH | Acids / metabolism* Amino Acid Sequence Biofilms* Cell Movement / physiology Crystallography, X-Ray DNA-Binding Proteins / chemistry Dimerization Escherichia coli / metabolism* Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Hydrogen Peroxide / metabolism Indoles / metabolism Models, Molecular Molecular Sequence Data Multigene Family Oligonucleotide Array Sequence Analysis Oxidants / metabolism Protein Structure, Quaternary* Sequence Alignment Thermolysin / chemistry |
| IF | 4.76 |
| 引用数 | 53 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) |