RRC ID 3318
Author Lee J, Page R, García-Contreras R, Palermino JM, Zhang XS, Doshi O, Wood TK, Peti W.
Title Structure and function of the Escherichia coli protein YmgB: a protein critical for biofilm formation and acid-resistance.
Journal J Mol Biol
Abstract The Escherichia coli gene cluster ymgABC was identified in transcriptome studies to have a role in biofilm development and stability. In this study, we showed that YmgB represses biofilm formation in rich medium containing glucose, decreases cellular motility, and protects the cell from acid indicating that YmgB has a major role in acid-resistance in E. coli. Our data show that these phenotypes are potentially mediated through interactions with the important cell signal indole. In addition, gel mobility-shift assays suggest that YmgB may be a non-specific DNA-binding protein. Using nickel-enrichment DNA microarrays, we showed that YmgB binds, either directly or indirectly, via a probable ligand, genes important for biofilm formation. To advance our understanding of the function of YmgB, we used X-ray crystallography to solve the structure of the protein to 1.8 A resolution. YmgB is a biological dimer that is structurally homologous to the E. coli gene regulatory protein Hha, despite having only 5% sequence identity. This supports our DNA microarray data showing that YmgB is a gene regulatory protein. Therefore, this protein, which clearly has a critical role in acid-resistance in E. coli, has been renamed as AriR for regulator of acid resistance influenced by indole.
Volume 373(1)
Pages 11-26
Published 2007-10-12
DOI 10.1016/j.jmb.2007.07.037
PII S0022-2836(07)00964-3
PMID 17765265
PMC PMC2185545
MeSH Acids / metabolism* Amino Acid Sequence Biofilms* Cell Movement / physiology Crystallography, X-Ray DNA-Binding Proteins / chemistry Dimerization Escherichia coli / metabolism* Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Hydrogen Peroxide / metabolism Indoles / metabolism Models, Molecular Molecular Sequence Data Multigene Family Oligonucleotide Array Sequence Analysis Oxidants / metabolism Protein Structure, Quaternary* Sequence Alignment Thermolysin / chemistry
IF 4.76
Times Cited 53
Prokaryotes E. coli ME9062(BW25113)