論文 - 詳細
| RRC ID | 3319 |
|---|---|
| 著者 | Lee J, Bansal T, Jayaraman A, Bentley WE, Wood TK. |
| タイトル | Enterohemorrhagic Escherichia coli biofilms are inhibited by 7-hydroxyindole and stimulated by isatin. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Since indole is present at up to 500 microM in the stationary phase and is an interspecies biofilm signal (J. Lee, A. Jayaraman, and T. K. Wood, BMC Microbiol. 7:42, 2007), we investigated hydroxyindoles as biofilm signals and found them also to be nontoxic interspecies biofilm signals for enterohemorrhagic Escherichia coli O157:H7 (EHEC), E. coli K-12, and Pseudomonas aeruginosa. The genetic basis of EHEC biofilm formation was also explored, and notably, virulence genes in biofilm cells were repressed compared to those in planktonic cells. In Luria-Bertani medium (LB) on polystyrene with quiescent conditions, 7-hydroxyindole decreased EHEC biofilm formation 27-fold and decreased K-12 biofilm formation 8-fold without affecting the growth of planktonic cells. 5-Hydroxyindole also decreased biofilm formation 11-fold for EHEC and 6-fold for K-12. In contrast, isatin (indole-2,3-dione) increased biofilm formation fourfold for EHEC, while it had no effect for K-12. When continuous-flow chambers were used, confocal microscopy revealed that EHEC biofilm formation was reduced 6-fold by indole and 10-fold by 7-hydroxyindole in LB. Whole-transcriptome analysis revealed that isatin represses indole synthesis by repressing tnaABC 7- to 37-fold in EHEC, and extracellular indole levels were found to be 20-fold lower. Furthermore, isatin repressed the AI-2 transporters lsrABCDFGKR, while significantly inducing the flagellar genes flgABCDEFGHIJK and fliAEFGILMNOPQ (which led to a 50% increase in motility). 7-Hydroxyindole induces the biofilm inhibitor/stress regulator ycfR and represses cysADIJPU/fliC (which led to a 50% reduction in motility) and purBCDEFHKLMNRT. Isogenic mutants showed that 7-hydroxyindole inhibits E. coli biofilm through cysteine metabolism. 7-Hydroxyindole (500 microM) also stimulates P. aeruginosa PAO1 biofilm formation twofold; therefore, hydroxyindoles are interspecies bacterial signals, and 7-hydroxyindole is a potent EHEC biofilm inhibitor. |
| 巻・号 | 73(13) |
| ページ | 4100-9 |
| 公開日 | 2007-7-1 |
| DOI | 10.1128/AEM.00360-07 |
| PII | AEM.00360-07 |
| PMID | 17483266 |
| PMC | PMC1932762 |
| MeSH | Base Sequence Biofilms / drug effects* Biofilms / growth & development Cysteine / metabolism DNA Primers / genetics DNA, Bacterial / genetics Escherichia coli K12 / drug effects Escherichia coli K12 / genetics Escherichia coli K12 / growth & development Escherichia coli O157 / drug effects* Escherichia coli O157 / genetics Escherichia coli O157 / pathogenicity Escherichia coli O157 / physiology Gene Expression / drug effects Genes, Bacterial / drug effects Humans In Vitro Techniques Indoles / pharmacology* Isatin / pharmacology* Movement / drug effects Mutation Pseudomonas aeruginosa / drug effects Pseudomonas aeruginosa / genetics Pseudomonas aeruginosa / growth & development Signal Transduction |
| IF | 4.016 |
| 引用数 | 136 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) |