Reference - Detail
| RRC ID | 33219 |
|---|---|
| Author | Sokol NS, Ambros V. |
| Title | Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. |
| Journal | Genes Dev |
| Abstract |
Although hundreds of evolutionarily conserved microRNAs have been discovered, the functions of most remain unknown. Here, we describe the embryonic spatiotemporal expression profile, transcriptional regulation, and loss-of-function phenotype of Drosophila miR-1 (DmiR-1). DmiR-1 RNA is highly expressed throughout the mesoderm of early embryos and subsequently in somatic, visceral, and pharyngeal muscles, and the dorsal vessel. The expression of DmiR-1 is controlled by the Twist and Mef2 transcription factors. DmiR-1KO mutants, generated using ends-in gene targeting, die as small, immobilized second instar larvae with severely deformed musculature. This lethality is rescued when a DmiR-1 transgene is expressed specifically in the mesoderm and muscle. Strikingly, feeding triggers DmiR-1KO-associated paralysis and death; starved first instar DmiR-1KO larvae are essentially normal. Thus, DmiR-1 is not required for the formation or physiological function of the larval musculature, but is required for the dramatic post-mitotic growth of larval muscle. |
| Volume | 19(19) |
| Pages | 2343-54 |
| Published | 2005-10-1 |
| DOI | 10.1101/gad.1356105 |
| PII | gad.1356105 |
| PMID | 16166373 |
| PMC | PMC1240043 |
| MeSH | Animals Drosophila Drosophila Proteins / metabolism* Gene Deletion Gene Expression Regulation, Developmental / physiology* Larva / growth & development Mesoderm / metabolism* MicroRNAs / biosynthesis* MicroRNAs / genetics Mitosis / physiology Muscle Development / physiology* Muscles / embryology Myogenic Regulatory Factors / metabolism Transcription, Genetic / physiology |
| IF | 9.527 |
| Times Cited | 296 |
| WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 21 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |