Reference - Detail
| RRC ID | 33283 |
|---|---|
| Author | Kaneko T, Mashimo T. |
| Title | Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection. |
| Journal | Methods Mol Biol |
| Abstract |
Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9. |
| Volume | 1239 |
| Pages | 307-15 |
| Published | 2015-1-1 |
| DOI | 10.1007/978-1-4939-1862-1_18 |
| PMID | 25408415 |
| MeSH | Animals Embryo Transfer / methods Embryo, Mammalian* Endonucleases / genetics* Endonucleases / metabolism* Female Fertilization in Vitro / methods Gene Knock-In Techniques* Gene Knockout Techniques* Genetic Engineering / methods* Genotyping Techniques Male Mice Microinjections / methods* RNA, Messenger / administration & dosage RNA, Messenger / genetics Rats Rodentia |
| Times Cited | 13 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Rats | F344/Stm(strainID=266) |