RRC ID |
33289
|
著者 |
Isono K, Endo TA, Ku M, Yamada D, Suzuki R, Sharif J, Ishikura T, Toyoda T, Bernstein BE, Koseki H.
|
タイトル |
SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing.
|
ジャーナル |
Dev Cell
|
Abstract |
The Polycomb-group (PcG) repressive complex-1 (PRC1) forms microscopically visible clusters in nuclei; however, the impact of this cluster formation on transcriptional regulation and the underlying mechanisms that regulate this process remain obscure. Here, we report that the sterile alpha motif (SAM) domain of a PRC1 core component Phc2 plays an essential role for PRC1 clustering through head-to-tail macromolecular polymerization, which is associated with stable target binding of PRC1/PRC2 and robust gene silencing activity. We propose a role for SAM domain polymerization in this repression by two distinct mechanisms: first, through capturing and/or retaining PRC1 at the PcG targets, and second, by strengthening the interactions between PRC1 and PRC2 to stabilize transcriptional repression. Our findings reveal a regulatory mechanism mediated by SAM domain polymerization for PcG-mediated repression of developmental loci that enables a robust yet reversible gene repression program during development.
|
巻・号 |
26(6)
|
ページ |
565-77
|
公開日 |
2013-9-30
|
DOI |
10.1016/j.devcel.2013.08.016
|
PII |
S1534-5807(13)00503-0
|
PMID |
24091011
|
MeSH |
Animals
Cell Line
Cell Nucleus / metabolism
Chromatin / metabolism
Cytoplasm / metabolism
Epigenetic Repression
Fibroblasts / metabolism
Gene Silencing*
Mice
Polycomb Repressive Complex 2 / chemistry
Polycomb Repressive Complex 2 / genetics
Polycomb Repressive Complex 2 / metabolism*
Polycomb-Group Proteins / genetics
Polycomb-Group Proteins / metabolism*
Protein Binding
Protein Multimerization*
Protein Structure, Tertiary
Transcription, Genetic
|
IF |
10.092
|
引用数 |
119
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
遺伝子材料 |
pGFP-Phc2 (RDB12787)
pGFP-Phc2 L307R (RDB12788) |