論文 - 詳細
| RRC ID | 33289 |
|---|---|
| 著者 | Isono K, Endo TA, Ku M, Yamada D, Suzuki R, Sharif J, Ishikura T, Toyoda T, Bernstein BE, Koseki H. |
| タイトル | SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. |
| ジャーナル | Dev Cell |
| Abstract |
The Polycomb-group (PcG) repressive complex-1 (PRC1) forms microscopically visible clusters in nuclei; however, the impact of this cluster formation on transcriptional regulation and the underlying mechanisms that regulate this process remain obscure. Here, we report that the sterile alpha motif (SAM) domain of a PRC1 core component Phc2 plays an essential role for PRC1 clustering through head-to-tail macromolecular polymerization, which is associated with stable target binding of PRC1/PRC2 and robust gene silencing activity. We propose a role for SAM domain polymerization in this repression by two distinct mechanisms: first, through capturing and/or retaining PRC1 at the PcG targets, and second, by strengthening the interactions between PRC1 and PRC2 to stabilize transcriptional repression. Our findings reveal a regulatory mechanism mediated by SAM domain polymerization for PcG-mediated repression of developmental loci that enables a robust yet reversible gene repression program during development. |
| 巻・号 | 26(6) |
| ページ | 565-77 |
| 公開日 | 2013-9-30 |
| DOI | 10.1016/j.devcel.2013.08.016 |
| PII | S1534-5807(13)00503-0 |
| PMID | 24091011 |
| MeSH | Animals Cell Line Cell Nucleus / metabolism Chromatin / metabolism Cytoplasm / metabolism Epigenetic Repression Fibroblasts / metabolism Gene Silencing* Mice Polycomb Repressive Complex 2 / chemistry Polycomb Repressive Complex 2 / genetics Polycomb Repressive Complex 2 / metabolism* Polycomb-Group Proteins / genetics Polycomb-Group Proteins / metabolism* Protein Binding Protein Multimerization* Protein Structure, Tertiary Transcription, Genetic |
| IF | 10.092 |
| 引用数 | 119 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pGFP-Phc2 (RDB12787) pGFP-Phc2 L307R (RDB12788) |