Reference - Detail
|Author||Pérez JM, Calderón IL, Arenas FA, Fuentes DE, Pradenas GA, Fuentes EL, Sandoval JM, Castro ME, Elías AO, Vásquez CC.|
|Title||Bacterial toxicity of potassium tellurite: unveiling an ancient enigma.|
Biochemical, genetic, enzymatic and molecular approaches were used to demonstrate, for the first time, that tellurite (TeO(3) (2-)) toxicity in E. coli involves superoxide formation. This radical is derived, at least in part, from enzymatic TeO(3) (2-) reduction. This conclusion is supported by the following observations made in K(2)TeO(3)-treated E. coli BW25113: i) induction of the ibpA gene encoding for the small heat shock protein IbpA, which has been associated with resistance to superoxide, ii) increase of cytoplasmic reactive oxygen species (ROS) as determined with ROS-specific probe 2'7'-dichlorodihydrofluorescein diacetate (H(2)DCFDA), iii) increase of carbonyl content in cellular proteins, iv) increase in the generation of thiobarbituric acid-reactive substances (TBARs), v) inactivation of oxidative stress-sensitive [Fe-S] enzymes such as aconitase, vi) increase of superoxide dismutase (SOD) activity, vii) increase of sodA, sodB and soxS mRNA transcription, and viii) generation of superoxide radical during in vitro enzymatic reduction of potassium tellurite.
|MeSH||Aconitate Hydratase / antagonists & inhibitors Bacterial Proteins / biosynthesis Bacterial Proteins / genetics Cold Shock Proteins and Peptides Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / antagonists & inhibitors Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Fluoresceins / analysis Gene Expression Regulation, Bacterial / drug effects Heat-Shock Proteins / biosynthesis Heat-Shock Proteins / genetics Oxidation-Reduction RNA, Bacterial / biosynthesis RNA, Messenger / biosynthesis Reactive Oxygen Species / metabolism Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / genetics Superoxide Dismutase / biosynthesis Superoxide Dismutase / genetics Superoxides / metabolism Tellurium / toxicity* Thiobarbituric Acid Reactive Substances / analysis Trans-Activators / biosynthesis Trans-Activators / genetics|
|Prokaryotes E. coli||ME9062(BW25113)|