論文 - 詳細
| RRC ID | 3334 |
|---|---|
| 著者 | Sakai T, Nakamura N, Umitsuki G, Nagai K, Wachi M. |
| タイトル | Increased production of pyruvic acid by Escherichia coli RNase G mutants in combination with cra mutations. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis revealed that simultaneous defects of cra and ilvIH, encoding a transcriptional regulator of glycolysis/gluconeogenesis and one of isozymes of acetohydroxy acid synthase, respectively, were required for this phenomenon to occur. The results of additional experiments presented here indicate that the RNase G mutation, in combination with cra mutation, caused the increased production of pyruvic acid from glucose, which was then preferentially converted to valine due to the ilvIH mutation, resulting in depletion of isoleucine. In fact, the rng cra double mutant produced increased amount of pyruvate in the medium. These results suggest that the RNase G mutation could be applied in the breeding of producer strains of pyruvate and its derivatives such as valine. |
| 巻・号 | 76(1) |
| ページ | 183-92 |
| 公開日 | 2007-8-1 |
| DOI | 10.1007/s00253-007-1006-9 |
| PMID | 17483940 |
| MeSH | Acetolactate Synthase / genetics Bacterial Proteins / genetics Culture Media Endoribonucleases / genetics Endoribonucleases / metabolism* Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Glucose Glycolysis Isoenzymes Mutation Pyruvic Acid / metabolism* Regulatory Elements, Transcriptional / physiology Repressor Proteins / genetics Valine / metabolism |
| IF | 3.53 |
| 引用数 | 15 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7986(MG1655) |