RRC ID 33355
Author Oh K, Matsumoto T, Yamagami A, Ogawa A, Yamada K, Suzuki R, Sawada T, Fujioka S, Yoshizawa Y, Nakano T.
Title YCZ-18 is a new brassinosteroid biosynthesis inhibitor.
Journal PLoS ONE
Abstract Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor, YCZ-18. Quantitative analysis the endogenous levels of BRs in Arabidopsis indicated that YCZ-18 significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of YCZ-18to purified recombinant CYP90D1 indicated that YCZ-18 induced a typical type II binding spectrum with a Kd value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with YCZ-18 treatment of Arabidopsis indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation.
Volume 10(3)
Pages e0120812
Published 2015
DOI 10.1371/journal.pone.0120812
PII PONE-D-14-30103
PMID 25793645
PMC PMC4368189
MeSH Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / metabolism Biosynthetic Pathways / drug effects* Brassinosteroids / biosynthesis* Cytochrome P-450 Enzyme System / metabolism Dioxoles / chemistry Dioxoles / pharmacology* Gene Expression Regulation, Plant / drug effects Phenotype Protein Binding Triazoles / chemistry Triazoles / pharmacology*
IF 2.766
Times Cited 2
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda13118