Reference - Detail
RRC ID | 33357 |
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Author | Hiromi Y, Okamoto H, Gehring WJ, Hotta Y. |
Title | Germline transformation with Drosophila mutant actin genes induces constitutive expression of heat shock genes. |
Journal | Cell |
Abstract |
Two Drosophila mutants KM75 and HH5, which are mutated in the act88F actin gene specific for the indirect flight muscles (IFM), synthesize heat shock proteins (hsps) constitutively in a tissue-specific manner. We have introduced cloned mutant act88F genes into a strain containing the wild-type act88F allele by P-element-mediated transformation. Flies transformed with a 4.05 kb KM75 act88F gene fragment encoding the p42 actin variant express both p42 and hsps specifically in the IFM. Using normal/mutant chimeric genes, the mutation sites of KM75 and HH5 were mapped within the sequence encoding the last 72 amino acids of actin. An in vitro mutated gene encoding a protein that lacks the 72 carboxy-terminal amino acids also induces constitutive hsp synthesis. |
Volume | 44(2) |
Pages | 293-301 |
Published | 1986-1-31 |
DOI | 10.1016/0092-8674(86)90763-4 |
PII | 0092-8674(86)90763-4 |
PMID | 3080247 |
MeSH | Actins / genetics* Animals Base Sequence Drosophila melanogaster / genetics* Flight, Animal Gene Expression Regulation Heat-Shock Proteins / genetics* Molecular Weight Muscles / physiology Mutation Tissue Distribution Transformation, Genetic beta-Galactosidase / genetics |
IF | 38.637 |
Times Cited | 86 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
Drosophila |