RRC ID 3336
著者 Smith LK, Gomez MJ, Shatalin KY, Lee H, Neyfakh AA.
タイトル Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
ジャーナル Genome Biol
Abstract We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.
巻・号 8(5)
ページ R87
公開日 2007-1-1
DOI 10.1186/gb-2007-8-5-r87
PII gb-2007-8-5-r87
PMID 17519022
PMC PMC1929150
MeSH Anti-Bacterial Agents / pharmacology Bacteria / drug effects Bacteria / genetics Bacteria / growth & development* Chloramphenicol / pharmacology Gene Deletion Gene Silencing* Genes, Bacterial / physiology* Genome, Bacterial Genomics / methods
IF 10.806
引用数 7
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) ME9062(BW25113)