Reference - Detail
| RRC ID | 3336 |
|---|---|
| Author | Smith LK, Gomez MJ, Shatalin KY, Lee H, Neyfakh AA. |
| Title | Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes. |
| Journal | Genome Biol |
| Abstract |
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies. |
| Volume | 8(5) |
| Pages | R87 |
| Published | 2007-1-1 |
| DOI | 10.1186/gb-2007-8-5-r87 |
| PII | gb-2007-8-5-r87 |
| PMID | 17519022 |
| PMC | PMC1929150 |
| MeSH | Anti-Bacterial Agents / pharmacology Bacteria / drug effects Bacteria / genetics Bacteria / growth & development* Chloramphenicol / pharmacology Gene Deletion Gene Silencing* Genes, Bacterial / physiology* Genome, Bacterial Genomics / methods |
| IF | 10.806 |
| Times Cited | 7 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | ME9062(BW25113) |