RRC ID 33365
著者 Hohn B, Collins J.
タイトル A small cosmid for efficient cloning of large DNA fragments.
ジャーナル Gene
Abstract The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described. It can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI (also BglII, BclI, Sau3A and MboI), SalI (also XhoI and AvaI), EcaI and PstI. Hybrid cosmids containing inserts in the size range of 40 kb are packaged in vitro and transduced with an efficiency of 5 X 10(4) - 5 X 10(5) clones/microgram of insert DNA. Prefractionation of the DNA fragments to be cloned into 40 kb sized fragments ensures the cloning of contiguous stretches of DNA. Proteins produced in vitro by the cosmid pHC79 are identical to the ones produced by its pBR322 parent.
巻・号 11(3-4)
ページ 291-8
公開日 1980-11-1
DOI 10.1016/0378-1119(80)90069-4
PII 0378-1119(80)90069-4
PMID 6260575
MeSH Bacteriophage lambda / genetics Cloning, Molecular / methods* DNA Restriction Enzymes Escherichia coli / genetics Genetic Vectors* Molecular Weight Plasmids Proteins / genetics
IF 2.984
引用数 779
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) pHC79