Reference - Detail
| RRC ID | 33367 |
|---|---|
| Author | Penfold RJ, Pemberton JM. |
| Title | An improved suicide vector for construction of chromosomal insertion mutations in bacteria. |
| Journal | Gene |
| Abstract |
We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded pi protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for alpha-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides. |
| Volume | 118(1) |
| Pages | 145-6 |
| Published | 1992-9-1 |
| DOI | 10.1016/0378-1119(92)90263-o |
| PII | 0378-1119(92)90263-O |
| PMID | 1511879 |
| MeSH | Bacterial Proteins / genetics* Chromosomes, Bacterial DNA Helicases* DNA, Recombinant / genetics DNA-Binding Proteins* Genetic Vectors / genetics* Mutagenesis, Insertional / methods* Replicon / genetics Rhodobacter sphaeroides / genetics* Trans-Activators* |
| IF | 2.984 |
| Times Cited | 235 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | pJP5603 pJP5608 |