RRC ID |
33376
|
Author |
Jain C.
|
Title |
New improved lacZ gene fusion vectors.
|
Journal |
Gene
|
Abstract |
New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background beta-galactosidase (beta Gal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
|
Volume |
133(1)
|
Pages |
99-102
|
Published |
1993-10-29
|
DOI |
10.1016/0378-1119(93)90231-q
|
PII |
0378-1119(93)90231-Q
|
PMID |
8224902
|
MeSH |
Base Sequence
Cloning, Molecular / methods*
DNA, Recombinant
Genetic Vectors*
Lac Operon*
Molecular Sequence Data
Plasmids
Restriction Mapping
|
IF |
2.984
|
Times Cited |
15
|
WOS Category
|
GENETICS & HEREDITY
|
Resource |
Prokaryotes E. coli |
placZ1
placZ1a
placZ2
placZ3
placZY1
placZY1a
placZY2
placZY3 |