Reference - Detail
| RRC ID | 33377 |
|---|---|
| Author | Wülfing C, Plückthun A. |
| Title | A versatile and highly repressible Escherichia coli expression system based on invertible promoters: expression of a gene encoding a toxic product. |
| Journal | Gene |
| Abstract |
A very flexible and tightly regulatable expression system has been constructed. It uses the principle of invertible promoters [Podhajska et al., Gene 40 (1985) 163-168]. Here, we describe the construction of a plasmid that provides the integrase, which causes promoter inversion in a tightly regulated fashion, as well as modified plasmids carrying the invertible module. The way the integrase is provided on a separate plasmid closely mimicks expression of the integrase from a lambda lysogen. Thus, the flexibility of the original system is considerably extended by making it strain-independent without compromising the tight regulation. We present the expression of a single-chain T-cell receptor fragment as an example of application, in order to illustrate the properties of this expression system. |
| Volume | 136(1-2) |
| Pages | 199-203 |
| Published | 1993-12-22 |
| DOI | 10.1016/0378-1119(93)90464-e |
| PII | 0378-1119(93)90464-E |
| PMID | 8294003 |
| MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular / methods* DNA Nucleotidyltransferases / genetics Escherichia coli / genetics* Integrases Molecular Sequence Data Plasmids Promoter Regions, Genetic* Receptors, Antigen, T-Cell / genetics |
| IF | 2.984 |
| Times Cited | 16 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pCW107 |