Reference - Detail
RRC ID | 33378 |
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Author | Cha J, Bishai W, Chandrasegaran S. |
Title | New vectors for direct cloning of PCR products. |
Journal | Gene |
Abstract |
We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency. |
Volume | 136(1-2) |
Pages | 369-70 |
Published | 1993-12-22 |
DOI | 10.1016/0378-1119(93)90498-r |
PII | 0378-1119(93)90498-R |
PMID | 8294034 |
MeSH | Bacteriophage M13 / genetics Base Sequence Cloning, Molecular / methods* DNA Genetic Vectors* Molecular Sequence Data Polymerase Chain Reaction* |
IF | 2.984 |
Times Cited | 35 |
WOS Category | GENETICS & HEREDITY |
Resource | |
Prokaryotes E. coli | pCBC2 pCBC1 |