Reference - Detail
|Title||pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.|
99%) and did not require purification of the vector fragment prior to cloning. The high-copy-number vector pKSS offers a multitude of restriction sites and all of the features for analysis of cloned fragments that stem from the cloning vector pBluescript (Stratagene, La Jolla, CA, USA). Thus, pKSS represents a valuable alternative to previously reported positive-selection vectors; it should prove particularly useful for cloning when expecting a high fraction of cells transformed with non-recombinant vector, and for construction of DNA libraries.
|MeSH||Alleles Base Sequence Cloning, Molecular / methods* Escherichia coli / metabolism* Fenclonine / metabolism Genetic Vectors* Molecular Sequence Data Phenylalanine-tRNA Ligase / biosynthesis Phenylalanine-tRNA Ligase / genetics* Phenylalanine-tRNA Ligase / metabolism Plasmids* Recombinant Fusion Proteins / biosynthesis Recombinant Proteins / biosynthesis* Restriction Mapping Substrate Specificity beta-Galactosidase / biosynthesis|
|WOS Category||GENETICS & HEREDITY|
|Prokaryotes E. coli||pKSS|