RRC ID 33381
Author Kast P.
Title pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.
Journal Gene
Abstract 99%) and did not require purification of the vector fragment prior to cloning. The high-copy-number vector pKSS offers a multitude of restriction sites and all of the features for analysis of cloned fragments that stem from the cloning vector pBluescript (Stratagene, La Jolla, CA, USA). Thus, pKSS represents a valuable alternative to previously reported positive-selection vectors; it should prove particularly useful for cloning when expecting a high fraction of cells transformed with non-recombinant vector, and for construction of DNA libraries.
Volume 138(1-2)
Pages 109-14
Published 1994-1-28
PII 0378-1119(94)90790-0
PMID 8125286
MeSH Alleles Base Sequence Cloning, Molecular / methods* Escherichia coli / metabolism* Fenclonine / metabolism Genetic Vectors* Molecular Sequence Data Phenylalanine-tRNA Ligase / biosynthesis Phenylalanine-tRNA Ligase / genetics* Phenylalanine-tRNA Ligase / metabolism Plasmids* Recombinant Fusion Proteins / biosynthesis Recombinant Proteins / biosynthesis* Restriction Mapping Substrate Specificity beta-Galactosidase / biosynthesis
IF 2.498
Times Cited 63
Prokaryotes E. coli pKSS