Reference - Detail
| RRC ID | 33383 |
|---|---|
| Author | DeLaFuente A, Cisneros E, Talavera A. |
| Title | Restriction end-converting vectors with tandem repeated multiple cloning sites. |
| Journal | Gene |
| Abstract |
The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles. |
| Volume | 139(1) |
| Pages | 83-6 |
| Published | 1994-2-11 |
| DOI | 10.1016/0378-1119(94)90527-4 |
| PII | 0378-1119(94)90527-4 |
| PMID | 8112593 |
| MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular / methods* DNA Restriction Enzymes / metabolism Escherichia coli / enzymology Escherichia coli / genetics Genetic Vectors* Molecular Sequence Data Plasmids RNA, Messenger / chemistry RNA, Messenger / metabolism Repetitive Sequences, Nucleic Acid* Restriction Mapping* Substrate Specificity beta-Galactosidase / biosynthesis beta-Galactosidase / genetics* beta-Galactosidase / metabolism |
| IF | 2.984 |
| Times Cited | 5 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pTC181 pTC182 pTC191 pTC192 |