RRC ID 33389
Author Ahmed A.
Title Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors.
Journal Gene
Abstract When used in conjunction with double-origin vectors, the popular exonuclease III method can generate nested deletions from both ends of cloned DNA fragments. Such bidirectional deletions can be useful in sequencing long DNA fragments in both directions.
Volume 151(1-2)
Pages 97-101
Published 1994-12-30
DOI 10.1016/0378-1119(94)90637-8
PII 0378-1119(94)90637-8
PMID 7828911
MeSH Base Sequence Cloning, Molecular DNA / chemistry* DNA / metabolism Escherichia coli / enzymology Escherichia coli / genetics* Exodeoxyribonucleases* Genetic Techniques Genetic Vectors* Molecular Sequence Data Oligodeoxyribonucleotides / chemistry Oligodeoxyribonucleotides / isolation & purification* Restriction Mapping Sequence Deletion*
IF 2.984
Times Cited 2
Prokaryotes E. coli pDO192 pDO193