Reference - Detail
| RRC ID | 33389 |
|---|---|
| Author | Ahmed A. |
| Title | Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors. |
| Journal | Gene |
| Abstract |
When used in conjunction with double-origin vectors, the popular exonuclease III method can generate nested deletions from both ends of cloned DNA fragments. Such bidirectional deletions can be useful in sequencing long DNA fragments in both directions. |
| Volume | 151(1-2) |
| Pages | 97-101 |
| Published | 1994-12-30 |
| DOI | 10.1016/0378-1119(94)90637-8 |
| PII | 0378-1119(94)90637-8 |
| PMID | 7828911 |
| MeSH | Base Sequence Cloning, Molecular DNA / chemistry* DNA / metabolism Escherichia coli / enzymology Escherichia coli / genetics* Exodeoxyribonucleases* Genetic Techniques Genetic Vectors* Molecular Sequence Data Oligodeoxyribonucleotides / chemistry Oligodeoxyribonucleotides / isolation & purification* Restriction Mapping Sequence Deletion* |
| IF | 2.984 |
| Times Cited | 2 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | pDO192 pDO193 |