論文 - 詳細
| RRC ID | 33389 |
|---|---|
| 著者 | Ahmed A. |
| タイトル | Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors. |
| ジャーナル | Gene |
| Abstract |
When used in conjunction with double-origin vectors, the popular exonuclease III method can generate nested deletions from both ends of cloned DNA fragments. Such bidirectional deletions can be useful in sequencing long DNA fragments in both directions. |
| 巻・号 | 151(1-2) |
| ページ | 97-101 |
| 公開日 | 1994-12-30 |
| DOI | 10.1016/0378-1119(94)90637-8 |
| PII | 0378-1119(94)90637-8 |
| PMID | 7828911 |
| MeSH | Base Sequence Cloning, Molecular DNA / chemistry* DNA / metabolism Escherichia coli / enzymology Escherichia coli / genetics* Exodeoxyribonucleases* Genetic Techniques Genetic Vectors* Molecular Sequence Data Oligodeoxyribonucleotides / chemistry Oligodeoxyribonucleotides / isolation & purification* Restriction Mapping Sequence Deletion* |
| IF | 2.984 |
| 引用数 | 2 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pDO192 pDO193 |