Reference - Detail
| RRC ID | 33396 |
|---|---|
| Author | Nakano Y, Yoshida Y, Yamashita Y, Koga T. |
| Title | Construction of a series of pACYC-derived plasmid vectors. |
| Journal | Gene |
| Abstract |
A series of cloning vectors has been constructed from pACYC177 and pACYC184. These vectors contain a MCS, the lacZ alpha reporter gene, and P15A ori. Plasmids pMBL18, pMBL19 and pMBLcos express the bla gene encoding ampicillin resistance, while pMCL200 and pMCL210 contain the cat gene encoding chloramphenicol resistance. In addition, the cosmid pMBLcos was constructed with the P15A ori. These vectors are useful for cloning or subcloning genes which are unstable and/or toxic in high-copy-number vectors. |
| Volume | 162(1) |
| Pages | 157-8 |
| Published | 1995-8-30 |
| DOI | 10.1016/0378-1119(95)00320-6 |
| PII | 0378111995003206 |
| PMID | 7557406 |
| MeSH | Aggregatibacter actinomycetemcomitans / genetics Ampicillin Resistance / genetics Chloramphenicol Resistance / genetics Cloning, Molecular / methods* Genes, Reporter Genetic Vectors* Genomic Library Plasmids / genetics* |
| IF | 2.984 |
| Times Cited | 104 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pMBL18 pMBL19 pMCL200 pMCL210 |