Reference - Detail
| RRC ID | 33397 |
|---|---|
| Author | Becker A, Schmidt M, Jäger W, Pühler A. |
| Title | New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. |
| Journal | Gene |
| Abstract |
A set of antibiotic-resistance and promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions was constructed. The cassettes contain the aacC1 gene of transposon Tn1696 conferring resistance to gentamicin in a large variety of Gram- and Gram+ bacteria. In addition to the antibiotic-resistance gene, a promoterless Escherichia coli lacZ gene was included in the cassettes, allowing the determination of the transcriptional activity at the insertion site. The cassettes can be excised from a plasmid mediating ampicillin resistance by many commonly used restriction enzymes. The new constructs have been successfully used for mutagenesis and studies of gene transcription in Rhizobium meliloti. |
| Volume | 162(1) |
| Pages | 37-9 |
| Published | 1995-8-30 |
| DOI | 10.1016/0378-1119(95)00313-u |
| PII | 037811199500313U |
| PMID | 7557413 |
| MeSH | Acetyltransferases / genetics Base Sequence Drug Resistance, Microbial / genetics Genetic Markers Gentamicins / pharmacology Lac Operon Molecular Sequence Data Mutagenesis, Insertional / methods* RNA / biosynthesis* Selection, Genetic* Transcription, Genetic* |
| IF | 2.984 |
| Times Cited | 135 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | pAB1001 pAB1002 pAB2001 pAB2002 pMS246 pMS252 pMS255 pMS266 pMS272 |