論文 - 詳細
| RRC ID | 33406 |
|---|---|
| 著者 | Tsao KL, DeBarbieri B, Michel H, Waugh DS. |
| タイトル | A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli. |
| ジャーナル | Gene |
| Abstract |
A versatile plasmid vector was designed to direct the synthesis of recombinant proteins in either one of two forms that will be biotinylated in Escherichia coli with high efficiency at a single, unique site. The protein of interest can be produced with a peptide substrate for E. coli biotin holoenzyme synthetase (BirA) joined directly to its N terminus, or alternatively, as a fusion to the C terminus of a maltose-binding protein domain (MalE) with the peptide substrate on its N terminus. To maximize the yield of biotinylated protein, the vector is designed to express the substrate in a coupled translation arrangement with the enzyme. |
| 巻・号 | 169(1) |
| ページ | 59-64 |
| 公開日 | 1996-2-22 |
| DOI | 10.1016/0378-1119(95)00762-8 |
| PII | 0378111995007628 |
| PMID | 8635750 |
| MeSH | ATP-Binding Cassette Transporters* Amino Acid Sequence Base Sequence Biotin* Carrier Proteins / chemistry DNA Primers / chemistry Escherichia coli Escherichia coli Proteins* Genetic Vectors* Maltose-Binding Proteins Molecular Sequence Data Monosaccharide Transport Proteins* Periplasmic Binding Proteins* Recombinant Proteins / chemistry Recombinant Proteins / isolation & purification* Sulfurtransferases / metabolism |
| IF | 2.984 |
| 引用数 | 67 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pDW363 |