Reference - Detail
RRC ID | 33407 |
---|---|
Author | Kato J, Ikeda H. |
Title | Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli. |
Journal | Gene |
Abstract |
We have constructed two Escherichia coli mini-F plasmid vectors, pJK286 and pJK289, which have unique EcoRI, BamHI and HindIII sites downstream from the lac promoter. The mini-F vectors are useful for plasmid shuffling, with which we can efficiently carry out localized mutagenesis. |
Volume | 170(1) |
Pages | 141-2 |
Published | 1996-4-17 |
DOI | 10.1016/0378-1119(95)00865-9 |
PII | 0378111995008659 |
PMID | 8621075 |
MeSH | Chromosomes, Bacterial / genetics Cloning, Molecular / methods* DNA, Bacterial / genetics DNA, Recombinant / genetics Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Escherichia coli / genetics* F Factor / genetics* Gene Targeting Genetic Vectors / genetics* Lac Operon / genetics Promoter Regions, Genetic |
IF | 2.984 |
Times Cited | 21 |
WOS Category | GENETICS & HEREDITY |
Resource | |
Prokaryotes E. coli | pJK286 pJK289 |