Reference - Detail
RRC ID | 33418 |
---|---|
Author | Miller WG, Lindow SE. |
Title | An improved GFP cloning cassette designed for prokaryotic transcriptional fusions. |
Journal | Gene |
Abstract |
A new gfp cloning cassette designed for prokaryotic transcriptional fusions has been constructed. This cassette consists of gfp (containing the S65T 'red-shift' [Heim et al. (1995) Nature 373, 663-664] and F64L 'protein solubility' [Cormack et al. (1996) Gene 173, 33-38] mutations) flanked by convenient restriction sites, a translational enhancer, and a consensus ribosome binding site with an optimized spacer region. gfp fusion strains containing this cassette demonstrate from 40- to 80-fold greater fluorescence intensity than wild-type gfp fusion strains. Additionally, this cassette confers sufficient fluorescence to recipient cells to be used in low copy-number plasmids, with promoters conferring low levels of transcription, and in bacterial taxa other than Escherichia, such as Pseudomonas. |
Volume | 191(2) |
Pages | 149-53 |
Published | 1997-6-3 |
DOI | 10.1016/s0378-1119(97)00051-6 |
PII | S0378111997000516 |
PMID | 9218713 |
MeSH | Cloning, Molecular* Escherichia coli / genetics* Fluorescence Gene Expression Genes, Bacterial Genes, Reporter Genetic Vectors* Green Fluorescent Proteins Iron / pharmacology Luminescent Proteins / genetics* Luminescent Proteins / metabolism Mutation Promoter Regions, Genetic Protein Biosynthesis Pseudomonas / genetics* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Solubility Transcription, Genetic |
IF | 2.984 |
Times Cited | 183 |
WOS Category | GENETICS & HEREDITY |
Resource | |
Prokaryotes E. coli | pGreen pGreenTIR |