論文 - 詳細
| RRC ID | 33421 |
|---|---|
| 著者 | Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. |
| タイトル | Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. |
| ジャーナル | Gene |
| Abstract |
In vitro recombination techniques were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI. Survival of Escherichia coli strain X1776 containing pBR313 and pBR322 as a function of thymine and diaminopimelic acid (DAP) starvation and sensitivity to bile salts was found to be equivalent to the non-plasmid containing strain. Conjugal transfer of these plasmids in bi- and triparental matings were significantly reduced or undetectable relative to the plasmid ColE1. |
| 巻・号 | 2(2) |
| ページ | 95-113 |
| 公開日 | 1977-1-1 |
| PII | 0378-1119(77)90000-2 |
| PMID | 344137 |
| MeSH | Ampicillin / pharmacology Conjugation, Genetic DNA, Bacterial DNA, Recombinant Escherichia coli / genetics Plasmids* Recombination, Genetic* Tetracycline / pharmacology Transformation, Bacterial |
| IF | 2.984 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 459 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pBR322 |