| RRC ID |
33421
|
| 著者 |
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S.
|
| タイトル |
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
|
| ジャーナル |
Gene
|
| Abstract |
In vitro recombination techniques were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI. Survival of Escherichia coli strain X1776 containing pBR313 and pBR322 as a function of thymine and diaminopimelic acid (DAP) starvation and sensitivity to bile salts was found to be equivalent to the non-plasmid containing strain. Conjugal transfer of these plasmids in bi- and triparental matings were significantly reduced or undetectable relative to the plasmid ColE1.
|
| 巻・号 |
2(2)
|
| ページ |
95-113
|
| 公開日 |
1977-1-1
|
| PII |
0378-1119(77)90000-2
|
| PMID |
344137
|
| MeSH |
Ampicillin / pharmacology
Conjugation, Genetic
DNA, Bacterial
DNA, Recombinant
Escherichia coli / genetics
Plasmids*
Recombination, Genetic*
Tetracycline / pharmacology
Transformation, Bacterial
|
| IF |
2.984
|
| リソース情報 |
| 原核生物(大腸菌) |
pBR322 |
