| RRC ID |
33423
|
| Author |
Remaut E, Tsao H, Fiers W.
|
| Title |
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
|
| Journal |
Gene
|
| Abstract |
Improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid R1 and carry the strong leftward promoter (pL) of bacteriophage lambda. The activity of this promoter is controlled by a temperature-sensitive repressor, product of the phage gene cI cloned on a compatible plasmid. Heat induction leads to amplification of the plasmid copy number and at the same time turns on the promoter. At a short distance downstream from the promoter, unique EcoRI, BamHI, XbaI and HindIII sites are present. This system was used for high level expression of the T4 DNA-ligase gene; 3 h after induction the ligase amounted to about 20% of total cellular protein.
|
| Volume |
22(1)
|
| Pages |
103-13
|
| Published |
1983-4-1
|
| DOI |
10.1016/0378-1119(83)90069-0
|
| PII |
0378-1119(83)90069-0
|
| PMID |
6305768
|
| MeSH |
Bacteriophage lambda / genetics*
Escherichia coli / genetics*
Gene Amplification
Genetic Vectors*
Mutation
Operon
Plasmids*
Replicon
Species Specificity
Temperature
|
| IF |
2.984
|
| Times Cited |
340
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Prokaryotes E. coli |
pCP3
pCP40 |
