Reference - Detail
| RRC ID | 33430 |
|---|---|
| Author | Norrander J, Kempe T, Messing J. |
| Title | Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. |
| Journal | Gene |
| Abstract |
The restriction endonuclease cleavage sites for SphI and KpnI have been added to the lac cloning region of the phage vectors M13mp10 and M13mp11, using oligodeoxynucleotide-directed in vitro mutagenesis. Complementary deoxy 16-, 21- or 18-mers with the desired base changes were annealed to the M13mp DNA strand and extended with the Klenow fragment of DNA polymerase I. In adding these sites we have shown that this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes. |
| Volume | 26(1) |
| Pages | 101-6 |
| Published | 1983-12-1 |
| DOI | 10.1016/0378-1119(83)90040-9 |
| PII | 0378-1119(83)90040-9 |
| PMID | 6323249 |
| MeSH | Base Sequence Cloning, Molecular Coliphages / genetics* DNA Restriction Enzymes Genetic Vectors* Mutation* Oligodeoxyribonucleotides* Oligonucleotides* |
| IF | 2.984 |
| Times Cited | 2319 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 92 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | M13mp18 M13mp19 |