論文 - 詳細
| RRC ID | 33436 |
|---|---|
| 著者 | Rao RN. |
| タイトル | Construction and properties of plasmid pKC30, a pBR322 derivative containing the pL-N region of phage lambda. |
| ジャーナル | Gene |
| Abstract |
The phage lambda N gene, contained on a HindIII-BamHI lambda DNA fragment (lambda coordinates 38 695-34 500), was inserted into plasmid pBR322 and cloned in Escherichia coli containing a defective lambda prophage. The plasmid is called pKC30. lambda N-mediated pL transcription interfered with plasmid pKC30 maintenance. Plasmid pKC30 was stabilized (i) by repression of pL, (ii) by a nutL mutation, or (iii) by cloning an "N-unresponsive terminator" sequence downstream from N gene. |
| 巻・号 | 31(1-3) |
| ページ | 247-50 |
| 公開日 | 1984-11-1 |
| DOI | 10.1016/0378-1119(84)90216-6 |
| PII | 0378-1119(84)90216-6 |
| PMID | 6098529 |
| MeSH | Bacteriophage lambda / genetics* Cloning, Molecular Escherichia coli / genetics Gene Expression Regulation Genes, Viral* Genetic Vectors* Plasmids* Promoter Regions, Genetic Viral Proteins / genetics* |
| IF | 2.984 |
| 引用数 | 28 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pKC101 pKC37 |