Reference - Detail
| RRC ID | 33441 |
|---|---|
| Author | Gambill BD, Summers AO. |
| Title | Versatile mercury-resistant cloning and expression vectors. |
| Journal | Gene |
| Abstract |
Cloning vectors have been constructed employing two diverse replicons, IncQ and P15A. Both vectors confer resistance to kanamycin (Km) and mercuric ions (Hg2+). One of these vectors, pDG105, is a broad-host-range, nonconjugative, oligocopy IncQ plasmid, which is capable of transforming Escherichia coli, Acinetobacter calcoaceticus, and Pseudomonas putida. The second vector, pDG106, is a narrow-host-range, multicopy cloning vector compatible with pBR322. Both vectors contain unique cloning sites in the Km-resistance gene for HindIII, SmaI, and XhoI, as well as unique EcoRI and ScaI sites in the mer operon. Cloning into the EcoRI site in the mer operon results in the mercury "supersensitive" phenotype, easily detectable by replica plating. Insertion of the galK gene into the EcoRI site in the mer operon results in Hg2+-inducible galactokinase activity, demonstrating the application of these plasmids as regulated expression vectors. |
| Volume | 39(2-3) |
| Pages | 293-7 |
| Published | 1985-1-1 |
| DOI | 10.1016/0378-1119(85)90326-9 |
| PII | 0378-1119(85)90326-9 |
| PMID | 4092936 |
| MeSH | Biological Transport Cloning, Molecular / methods* Drug Resistance, Microbial Galactokinase / genetics Gene Expression Regulation Genetic Vectors* Mercury / toxicity* Operon Organomercury Compounds / toxicity* Replicon |
| IF | 2.984 |
| Times Cited | 23 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pDG106 |