RRC ID 33454
著者 Gibson TJ, Coulson AR, Sulston JE, Little PF.
タイトル Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency.
ジャーナル Gene
Abstract Transcription terminators have been included in a phage-lambda-replicon-based cosmid vector, Lorist2, to insulate vector genes against transcriptional interference from cloned insert DNA. DNA yields of recombinant clones containing Escherichia coli genomic DNA inserts are more even for Lorist2 than with its progenitor LoristB. However, the terminators provide only a partial reduction in the over-representation of r X DNA-containing clones generally observed in cosmid libraries of Caenorhabditis elegans DNA, suggesting that causes other than transcriptional readthrough into the vector contribute to this problem.
巻・号 53(2-3)
ページ 275-81
公開日 1987-1-1
DOI 10.1016/0378-1119(87)90016-3
PII 0378-1119(87)90016-3
PMID 3301536
MeSH Animals Caenorhabditis / genetics Cell Division Cloning, Molecular / methods* Cosmids* DNA Replication* Escherichia coli / genetics Genes, Regulator* Genetic Vectors* Promoter Regions, Genetic Terminator Regions, Genetic*
IF 2.984
引用数 85
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) Lorist2